Characterization and varied expression of a membrane-bound endo-beta-1,4-glucanase in hybrid poplar.
Identifieur interne : 003348 ( Main/Exploration ); précédent : 003347; suivant : 003349Characterization and varied expression of a membrane-bound endo-beta-1,4-glucanase in hybrid poplar.
Auteurs : Victoria J. Maloney [Canada] ; Shawn D. MansfieldSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2010.
Descripteurs français
- KwdFr :
- Arbres (croissance et développement), Cellulase (génétique), Cellulase (métabolisme), Cellulose (composition chimique), Cellulose (ultrastructure), Données de séquences moléculaires (MeSH), Glucides (analyse), Gènes de plante (MeSH), Interférence par ARN (MeSH), Lignanes (analyse), Paroi cellulaire (métabolisme), Populus (enzymologie), Populus (génétique), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (MeSH), Séquence d'acides aminés (MeSH), Végétaux génétiquement modifiés (enzymologie), Végétaux génétiquement modifiés (génétique), Xylose (composition chimique), Xylème (métabolisme).
- MESH :
- analyse : Glucides, Lignanes.
- composition chimique : Cellulose, Xylose.
- croissance et développement : Arbres.
- enzymologie : Populus, Végétaux génétiquement modifiés.
- génétique : Cellulase, Populus, Protéines végétales, Végétaux génétiquement modifiés.
- métabolisme : Cellulase, Paroi cellulaire, Protéines végétales, Xylème.
- ultrastructure : Cellulose, Données de séquences moléculaires, Gènes de plante, Interférence par ARN, Régulation de l'expression des gènes végétaux, Régulation négative, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Carbohydrates (analysis), Cell Wall (metabolism), Cellulase (genetics), Cellulase (metabolism), Cellulose (chemistry), Cellulose (ultrastructure), Down-Regulation (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Lignans (analysis), Molecular Sequence Data (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (genetics), Populus (enzymology), Populus (genetics), RNA Interference (MeSH), Trees (growth & development), Xylem (metabolism), Xylose (chemistry).
- MESH :
- chemical , analysis : Carbohydrates, Lignans.
- chemical , chemistry : Cellulose, Xylose.
- chemical , genetics : Cellulase, Plant Proteins.
- enzymology : Plants, Genetically Modified, Populus.
- genetics : Plants, Genetically Modified, Populus.
- growth & development : Trees.
- metabolism : Cell Wall, Cellulase, Plant Proteins, Xylem.
- chemical , ultrastructure : Cellulose.
- Amino Acid Sequence, Down-Regulation, Gene Expression Regulation, Plant, Genes, Plant, Molecular Sequence Data, RNA Interference.
Abstract
To understand better the intricacies of secondary cell wall biosynthesis in trees, we investigated changes in cellulose chemistry and ultrastructure manifested by the mis-regulation of the poplar membrane-bound beta-1,4-endoglucanase orthologous to KORRIGAN (AtKOR). We isolated the poplar KORRIGAN gene from hybrid poplar (Populus albaxgrandidentata; designated PaxgKOR) and created a self-complementary (hairpin) RNAi suppression construct using PCR products derived from the gene. Additionally, AtKOR was employed to generate transgenic poplar over-expressing KORRIGAN. It was found that down-regulation leads to moderate to severe defects in plant growth, an irregular xylem (irx) phenotype, and significantly impacts the ultrastructure of the cellulose synthesized. The RNAi-suppressed lines deposited significantly reduced quantities of a more highly crystalline cellulose, while the hemicellulose content and, more specifically, the xylose content increased. In addition, the amount of soluble sucrose in the leaves and xylem decreased. Conversely, the AtKOR transgenics did not significantly alter cell wall development or plant growth parameters, but it did impact the ultrastructure of the cellulose produced, generating trees with less crystalline cellulose and reduced xylose content.
DOI: 10.1111/j.1467-7652.2009.00483.x
PubMed: 20070872
Affiliations:
Links toward previous steps (curation, corpus...)
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Mansfield</name></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Carbohydrates (analysis)</term><term>Cell Wall (metabolism)</term><term>Cellulase (genetics)</term><term>Cellulase (metabolism)</term><term>Cellulose (chemistry)</term><term>Cellulose (ultrastructure)</term><term>Down-Regulation (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Lignans (analysis)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (enzymology)</term><term>Plants, Genetically Modified (genetics)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>RNA Interference (MeSH)</term><term>Trees (growth & development)</term><term>Xylem (metabolism)</term><term>Xylose (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (croissance et développement)</term><term>Cellulase (génétique)</term><term>Cellulase (métabolisme)</term><term>Cellulose (composition chimique)</term><term>Cellulose (ultrastructure)</term><term>Données de séquences moléculaires (MeSH)</term><term>Glucides (analyse)</term><term>Gènes de plante (MeSH)</term><term>Interférence par ARN (MeSH)</term><term>Lignanes (analyse)</term><term>Paroi cellulaire (métabolisme)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation négative (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Végétaux génétiquement modifiés (enzymologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Xylose (composition chimique)</term><term>Xylème (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbohydrates</term><term>Lignans</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulose</term><term>Xylose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cellulase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucides</term><term>Lignanes</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulose</term><term>Xylose</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cellulase</term><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Cellulase</term><term>Plant Proteins</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term><term>Paroi cellulaire</term><term>Protéines végétales</term><term>Xylème</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Cellulose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Down-Regulation</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>RNA Interference</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Cellulose</term><term>Données de séquences moléculaires</term><term>Gènes de plante</term><term>Interférence par ARN</term><term>Régulation de l'expression des gènes végétaux</term><term>Régulation négative</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To understand better the intricacies of secondary cell wall biosynthesis in trees, we investigated changes in cellulose chemistry and ultrastructure manifested by the mis-regulation of the poplar membrane-bound beta-1,4-endoglucanase orthologous to KORRIGAN (AtKOR). We isolated the poplar KORRIGAN gene from hybrid poplar (Populus albaxgrandidentata; designated PaxgKOR) and created a self-complementary (hairpin) RNAi suppression construct using PCR products derived from the gene. Additionally, AtKOR was employed to generate transgenic poplar over-expressing KORRIGAN. It was found that down-regulation leads to moderate to severe defects in plant growth, an irregular xylem (irx) phenotype, and significantly impacts the ultrastructure of the cellulose synthesized. The RNAi-suppressed lines deposited significantly reduced quantities of a more highly crystalline cellulose, while the hemicellulose content and, more specifically, the xylose content increased. In addition, the amount of soluble sucrose in the leaves and xylem decreased. 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We isolated the poplar KORRIGAN gene from hybrid poplar (Populus albaxgrandidentata; designated PaxgKOR) and created a self-complementary (hairpin) RNAi suppression construct using PCR products derived from the gene. Additionally, AtKOR was employed to generate transgenic poplar over-expressing KORRIGAN. It was found that down-regulation leads to moderate to severe defects in plant growth, an irregular xylem (irx) phenotype, and significantly impacts the ultrastructure of the cellulose synthesized. The RNAi-suppressed lines deposited significantly reduced quantities of a more highly crystalline cellulose, while the hemicellulose content and, more specifically, the xylose content increased. In addition, the amount of soluble sucrose in the leaves and xylem decreased. Conversely, the AtKOR transgenics did not significantly alter cell wall development or plant growth parameters, but it did impact the ultrastructure of the cellulose produced, generating trees with less crystalline cellulose and reduced xylose content.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Maloney</LastName><ForeName>Victoria J</ForeName><Initials>VJ</Initials><AffiliationInfo><Affiliation>Department of Wood Science, Faculty of Forestry, The University of British Columbia, Vancouver, BC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>01</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002241">Carbohydrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017705">Lignans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>A1TA934AKO</RegistryNumber><NameOfSubstance UI="D014994">Xylose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017705" MajorTopicYN="N">Lignans</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014994" MajorTopicYN="N">Xylose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20070872</ArticleId><ArticleId IdType="pii">PBI483</ArticleId><ArticleId IdType="doi">10.1111/j.1467-7652.2009.00483.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Maloney, Victoria J" sort="Maloney, Victoria J" uniqKey="Maloney V" first="Victoria J" last="Maloney">Victoria J. Maloney</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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